Additionally, spelt was observed to produce biomass comparable
The overall aim of this study was to examine the role of C/EBP overexpression in regulating the transcription of UCP1 in 3T3 L1 white preadipocytes to transform them to a more brown like cell phenotype.Tetracycline inducible (Tet on) lentiviral, adipose specific expression vectors for overexpressing C/EBP (or the control Luciferase GFP) gene were constructed with the pLenti6 lentiviral vector backbone with TRE tight and rtTA advance regulatory elements. In the absence of doxycycline there was low basal expression from the vectors and a dose dependent, doxycycline induced transient, adipose specific overexpression was observed in 3T3 L1 preadipocytes. Transduction of the pLenti6 positive control luciferase RFP vector was successfully achieved but the C/EBP or LucGFP vectors constructed failed to produce highly infectious lentiviral particles, possibly due to the large size of insert which challenged the limit of the pLenti6 vector backbone.
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